Evaluation of Toxoplasma gondii IgM and IgG Seropositivities in Serum Samples Sent from Pediatric and Adult Hematology/Oncology Outpatient Clinics

Ayfer Bakır, Mustafa Guney


Toxoplasmosis, despite its mild course in immunocompetent individuals, it may have a more severe course in immunosuppressed patients such as hematology and oncology patients. This study aimed to contribute to the importance of that issue by evaluating the seropositivity rates of Toxoplasma gondii (T. gondii) in Hematology and Oncology patients and sharing the data obtained.
Serum samples sent from pediatric and adult Hematology/Oncology outpatient clinic to the Microbiology Laboratory between January 2017 and August 2019 were analysed using Architect Toxo IgG and IgM Reagent Kit with chemiluminescent microparticular enzyme immunoassay method on Architect system.
A total of 673 patient samples belonging to 131 pediatric patients and 542 adult patients were analysed in the study. Median age of the study group was 29 (range: 0 - 83) and 53.5% of them were male. While T. gondii IgM positivity was 8% IgG positivity was 28.7%. T. gondii IgG seropositivities in male and female patients were 27.2% and 30.4% respectively (p = 0.44). T. gondii IgM seropositivities in pediatric and adult patients were 6.9% and 8.3% respectively and IgG seropositivities were 24.4% and 29.7% (p = 0.78 and p = 0.46 respectively). T. gondii IgM positivity was found as 8% and IgG positivity as 28.7% in the study group. In conclusion, data obtained from the study were in compliance with both domestic and foreign data and the highest rate of IgM and IgG seropositivity was found in patients with lymphoma.


ELISA; Hematological malignancy; Oncology; Seropositivity; Toxoplasma gondii; Turkey

Full Text:



Abdel Malek R, Wassef R, Rizk E, Sabry H, Tadros N, Boghdady A. (2018).Toxoplasmosis an Overlooked Disease: Seroprevalence in Cancer Patients. Asian Pac J Cancer Prev, 19(7):1987-1991.

Akduman Alasehir E, Yaman G. (2018) Evaluation of Toxoplasma gondii Seroprevalence Among Women of Childbearing Age Group in Istanbul. Okmeydanı Tıp Dergisi, 34(2), 158-162.

Aşcı Z, Akgün S. (2015). The Evaluation of Toxoplasma gondii (T.gondii) Serology Results Among Cases Who Admitted to the Serology Laboratory of a Hospital in Afyon City. Turkiye Parazitol Derg, 39(1): 9-12.

Botein, E.F., Darwish, A., El-Tantawy, N.L., El-Baz, R., Eid, M.I., & Shaltot, A.M. (2019). Serological and molecular screening of umbilical cord blood for Toxoplasma gondii infection. Transplant infectious disease : an official journal of the Transplantation Society, 21(4), e13117.

CDC-Toxoplasmosis [Internet]. [cited 2020 Jan 2]. Available from: https://www.cdc.gov/parasites/toxoplasmosis/index.html.

Chehrazi-Raffle, A., Luu, M., Yu, Z., Liou, F., Kittleson, M., Hamilton, M., & Kobashigawa, J. (2015). Toxoplasma gondii Serology and Outcomes After Heart Transplantation: Contention in the Literature. Transplantation proceedings, 47(6), 1949–1953.

Chintakuntlawar, A., Kidd, M., Al-Kali, A., Wilson, W., & Thompson, C.A. (2015). Toxoplasmosis in patients with hematologic malignancies. Leukemia & lymphoma, 56(2), 536–538.

Çiçek, A.Ç., Duygu, F., İnakçı, İ.H., Boyar, N., & Boyar, İ.H. (2012). Investigation of Toxoplasma gondii antibodies with ELISA among women of childbearing age in Şanlıurfa province: A three years evaluation. Journal of Clinical and Experimental Investigations, 3(1), 61-65.

Duran, İ., Nazik, S., Nazik, H., & Duran, Ş. (2017). Evaluation of toxoplasma and rubella seropositivity in pregnancy. Balıkesir Med J, 1(1), 22-25.

Huang, Y., Huang, Y., Chang, A., Wang, J., Zeng, X., & Wu, J. (2016). Is Toxoplasma Gondii Infection a Risk Factor for Leukemia? An Evidence-Based Meta-Analysis. Medical science monitor : international medical journal of experimental and clinical research, 22, 1547–1552.

Israelski, D.M., & Remington, J.S. (1993). Toxoplasmosis in the non-AIDS immunocompromised host. Current clinical topics in infectious diseases, 13, 322–356.

Kaplan M, Bayar Z, Aslan R, Arı N, Özavcı H, Aşçı Toraman Z. (2014) Toxoplasma Seropositivity in Patients Admitted to the Central Laboratory of Firat University Hospital with the Suspicion of Toxoplasmosis. F.Ü.Sağ.Bil.Tıp Derg, 28(1): 21 - 24.

Kasap, B., Öner, G., Küçük, M., Öztürk Turhan, N., Akın, M.N., Arıkan, S., & Dirgen Çaylak, S. (2017). Evaluation of toxoplasmosis, rubella, cytomegalovirus and hepatitis prevalence of pregnant women in Muğla. Tepecik Eğitim ve Araştırma Hastahanesi Dergisi, 27, 31-36.

Khan, A.H., & Noordin, R. (2020). Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 39(1), 19–30.

Kojima, M., Nakamura, N., Murayama, K., Igarashi, T., Matsumoto, M., Matsuda, H., Masawa, N., Miura, I., & Morita, Y. (2010). Reactive lymphoid hyperplasia with giant follicles associated with a posttherapeutic state of hematological malignancies. A report of eight cases. Tumori, 96(1), 143–148.

Lappalainen M, Hedman K. (2004). Serodiagnosis of toxoplasmosis. The impact of measurement of IgG avidity. Ann Ist Super Sanita, 40(1): 81-88.

Maciel, E., Siqueira, I., Queiroz, A.C., & Melo, A. (2000). Toxoplasma gondii myelitis in a patient with adult T-cell leukemia-lymphoma. Arquivos de neuro-psiquiatria, 58(4), 1107–1109.

Malatyalı, E., Yıldız, İ., Tileklioğlu, E., Ertabaklar, H., & Ertuğ, S. (2019). Retrospective Analysis of Toxoplasma gondii Serology Results from Adnan Menderes University Faculty of Medicine Parasitology Laboratory from 2007 to 2017. Türkiye Parazitolojii Dergisi, 43(1), 1.

Montoya J.G. (2002). Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. The Journal of infectious diseases, 185 Suppl 1, S73–S82.

Pereira KS, Franco RM, Leal DA. (2010). Transmission of toxoplasmosis (Toxoplasma gondii) by foods. Adv Food Nutr Res, 60:1-19.

Robert-Gangneux F, Dardé M.L. (2012). Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev, 25(2): 264-296.

Saadatnia, G., & Golkar, M. (2012). A review on human toxoplasmosis. Scandinavian journal of infectious diseases, 44(11): 805–814.

Senoglu, S., Yesilbag, Z., Aydin, Ö.A., Karaosmanoglu, H.K., & Yasar, K.K. (2018). HIV/AIDS Hastalarinda Toxoplasma gondu IgG Seroprevalansi/Toxoplasma gondu IgG Seroprevalence in Patients with HIV/AIDS. Turkish Journal of Parasitology, 42(3),175-180.

Su, C., Khan, A., Zhou, P., Majumdar, D., Ajzenberg, D., Dardé, M.L., Zhu, X.Q., Ajioka, J.W., Rosenthal, B.M., Dubey, J.P., & Sibley, L.D. (2012). Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages. Proceedings of the National Academy of Sciences of the United States of America, 109(15): 5844–5849.

Okyay, A.G., Karateke, A., Yula, E., Inci, M., Şilfeler, D.B., Motor, V.K., ... & Okur, M. (2013) Seroprevalance of Toxoplasma IgG Among Pregnant Women in The Provınce of Hatay and Contrıbutıon of Avıdıty Test to The Dıagnose. Journal of Turkish Society of Obstetric and Gynecology, 10:160-164.

Wang, Z.D., Liu, H.H., Ma, Z.X., Ma, H.Y., Li, Z.Y., Yang, Z.B., Zhu, X.Q., Xu, B., Wei, F., & Liu, Q. (2017). Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis. Frontiers in microbiology, 8: 389.


  • There are currently no refbacks.