Pengaruh Ekstrak Etanol Biji Jinten Hitam (Nigella Sativa) terhadap Ekspresi ET-1 Aorta pada Mencit Model Preeklampsia
DOI:
https://doi.org/10.31964/mltj.v2i1.37Keywords:
Preeklampsia, Biji jinten hitam (Nigella sativa), ET-1, AortaAbstract
Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p<0,05).these doses of 500 mg, 1000mg, 1500mg, and 2000mg, ethanol extract of black cumin seeds has ability to decrease the ET-1 Aorta in preeclampsia model mouse. dose of 1500mg/KgBB/day is the most optimum dosage to reduce when compared to another dosages. Ethanol extract of black cumin seeds (Nigella Sativa) can decrease the expression of ET-1 Aorta in preeclampsia model mouse, particulary at dose 2000mg/Kg/BB/day sequentially Keyword : Preeclampsia; black cumin seeds (Nigella Sativa); ET-1; Aorta . Abstrak: Penelitian ini bertujuan untuk menganalisa pengaruh pemberian ekstrak etanol biji jinten hitam(Nigella Sativa) terhadap ekspresi ET-1 Aorta pada mencit model preeclampsia. Desain Penelitian ini menggunakan eksprimental (experimental) dengan jenis rancangan posttest only control group design. Menggunakan mencit model preeklampsia. Penelitian ini dibagi menjadi 6 kelompok yaitu Kontrol negatif, kontrol positif(Model), Model+ekstrak biji jinten hitam 500 mg/KgBB/hari, 1000 mg/KgBB/hari, 1500 mg/KgBB/hari, dan 2000 mg/KgBB/hari, setelah dilakukan pembedahan pada mencit kemudian dilakukan pemeriksaan ekspresi ET-1 Aorta dengan metode Immunohistokimia. Data hasil pengamatan dianalisis dengan uji one way anova. Ada pengaruh pemberian ekstrak etanol biji jinten hitam terhadap ekspresi ET-1 Aorta pada mencit model preeklampsia (p<0,05). Dosis 500mg, 1000mg, 1500mg, dan 2000mg jinten hitam mempunyai kemampuan terhadap penurunan ekspresi ET-1 Aorta Dosis yang paling optimum menurunkan adalah dosis 2000mg/KgBB/hari dibanding dosis yang lain. Ekstrak etanol biji jinten hitam (Nigella Sativa) dapat menurunkan ekspresi ET-1 Aorta pada mencit model preeklampsia terutama pada dosis 2000mg/KgBB/hari. Kata Kunci: Preeklampsia; Biji jinten hitam (Nigella sativa); ET-1; AortaDownloads
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