Identification of Acute Respiratory Infection Patients Using RP2 Nested Multiplex PCR Test in Jakarta, Indonesia
DOI:
https://doi.org/10.31964/mltj.v8i2.519Abstract
Acute Respiratory Infection (ARI) is an acute respiratory disease caused by infectious organisms transmitted between humans. Viruses and bacteria generally cause the cause of ARI infection. Other viruses that can also cause ARI are Influenza, Adenovirus, Enterovirus, and Respiratory Syncytial Virus. This study aims to determine the causes of bacterial or viral ARI infection with RP2 Nested Multiplex RT-PCR. The research methodology is cross-sectional. The sample used was 50 people with purposive sampling technique in patients with ARI who examined bacteria and viruses using RP2 Nested Multiplex RT-PCR. The research was located at the Laboratory of the United States Embassy in Jakarta, Indonesia. The results showed that 26 (52%) men suffered more from ARI patients than 24 women (46%), with the most age group being children, 28 people (56%). The three symptoms that many patients in this study felt were that 40 patients (80%) experienced nasal congestion, 38 patients (76%) experienced fever, and 32 patients (64%) coughed. The results of the organisms in the RP2 nested multiplex RT-PCR examination showed that 100% of the causes of ARI were viruses with the highest prevalence (40%) originating from the Human Rhinovirus/Enterovirus. The reason for ARI in this research is from a virus (100%), so antibiotics are not needed for this patient.References
Amy L. Leber, Kathy Everhart, Judy A. Daly, Aubrey Hopper, Amanda Harrington, Paul Schreckenberger, Kathleen McKinley, Matthew Jones, Kristen Holmberg, B. K. (2018). Multicenter Evaluation of BioFire FilmArray Respiratory Panel 2 for Detection of Viruses and Bacteria in Nasopharyngeal Swab Samples. Journal of Clinical Microbiology, 56(6), 1–29. https://doi.org/10.1128/JCM.01945-17
BioNumerics. (2021). BIOFIRE® Respiratory 2.1 plus Panel - clinical diagnostics products | bioMérieux Clinical Diagnostics (Copyright 2007–2020,; Vol. 1, Issue December, pp. 1–55). BioFire Diagnostics, LLC. https://doi.org/BFR0000-8303-02 December 2020
Creager, H. M., Cabrera, B., Schnaubelt, A., Cox, J. L., Cushman-vokoun, A. M., Shakir, S. M., Tardif, K. D., Huang, M., Jerome, K. R., Greninger, A. L., Drobysheva, D., Spaulding, U., Rogatcheva, M., Bourzac, K. M., Hinrichs, S. H., Broadhurst, M. J., & Fey, P. D. (2020). Clinical evaluation of the BioFire ® Respiratory Panel 2 . 1 and detection of. Journal of Clinical Virology, 129(July), 104538. https://doi.org/10.1016/j.jcv.2020.104538
Drysdale, S. B., Mejias, A., & Ramilo, O. (2017). Rhinovirus–not just the common cold. Journal of Infection, 74, S41–S46. https://doi.org/10.1016/S0163-4453(17)30190-1
European Centre for Disease Prevention and Control (ECDC). (2022). Intensified circulation of respiratory syncytial virus ( RSV ) and associated hospital burden in the EU / EEA (Vol. 12, Issue December). https://www.ecdc.europa.eu/en/publications-data/intensified-circulation-respiratory-syncytial-virus-rsv-and-associated-hospital
Fitzner, J., Qasmieh, S., Mounts, A. W., Alexander, B., Besselaar, T., Briand, S., Brown, C., Clark, S., Dueger, E., & Gross, D. (2018). Revision of clinical case definitions: influenza-like illness and severe acute respiratory infection. Bulletin of the World Health Organization, 96(2), 122. https://doi.org/10.2471/BLT.17.194514
Green, D. A., Hitoaliaj, L., Kotansky, B., Campbell, S. M., & Peaper, D. R. (2016). Clinical utility of on-demand multiplex respiratory pathogen testing among adult outpatients. Journal of Clinical Microbiology, 54(12), 2950–2955. https://doi.org/10.1128/JCM.01579-16
Hanson, K. E., Azar, M. M., Banerjee, R., Chou, A., Colgrove, R. C., Ginocchio, C. C., Hayden, M. K., Holodiny, M., Jain, S., & Koo, S. (2020). Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA’s Diagnostics Committee. Clinical Infectious Diseases, 71(10), 2744–2751. https://doi.org/10.1093/cid/ciaa508
Hassen, S., Getachew, M., Eneyew, B., Keleb, A., Ademas, A., Berihun, G., Berhanu, L., Yenuss, M., Natnael, T., & Kebede, A. B. (2020). Determinants of acute respiratory infection (ARI) among under-five children in rural areas of Legambo District, South Wollo Zone, Ethiopia: A matched case–control study. International Journal of Infectious Diseases, 96, 688–695. https://doi.org/10.1016/j.ijid.2020.05.012
Heinonen, S., Jartti, T., Garcia, C., Oliva, S., Smitherman, C., Anguiano, E., de Steenhuijsen Piters, W. A. A., Vuorinen, T., Ruuskanen, O., & Dimo, B. (2016). Rhinovirus detection in symptomatic and asymptomatic children: value of host transcriptome analysis. American Journal of Respiratory and Critical Care Medicine, 193(7), 772–782. https://doi.org/10.1164/rccm.201504-0749OC
Kakuya, F., Kinebuchi, T., Okubo, H., & Matsuo, K. (2017). Comparison of oropharyngeal and nasopharyngeal swab specimens for the detection of Mycoplasma pneumoniae in children with lower respiratory tract infection. The Journal of Pediatrics, 189, 218–221. https://doi.org/10.1016/j.jpeds.2017.06.038
Kardos, P., Dinh, Q. T., Fuchs, K.-H., Gillissen, A., Klimek, L., Koehler, M., Sitter, H., & Worth, H. (2020). German Respiratory Society guidelines for diagnosis and treatment of adults suffering from acute, subacute and chronic cough. Respiratory Medicine, 170, 105939. https://doi.org/10.1016/j.rmed.2020.105939
Lee, J., Yoo, D., Ryu, S., Ham, S., Lee, K., Yeo, M., Min, K., & Yoon, C. (2019). Quantity, size distribution, and characteristics of cough-generated aerosol produced by patients with an upper respiratory tract infection. Aerosol and Air Quality Research, 19(4), 840–853. https://doi.org/10.4209/aaqr.2018.01.0031
Lutpiatina, L., Sulistyorini, L., Notobroto, H. B., Raya, R. P., Utama, R. D., & Thuraidah, A. (2022). Multilevel Analysis of Lifestyle and Household Environment for Toddlers With Symptoms of Acute Respiratory Infection (ARI) in Indonesia in 2007, 2012, and 2017. Global Pediatric Health, 9, 1–13. https://doi.org/10.1177/2333794X221078700
Ministry of Health of Republic Indonesia. (2018). Main Results of Basic Health Research in 2018. Main Results of Basic Health Research in 2018, 53(9), 1689–1699.
Noviello, S., & Huang, D. B. (2019). The basics and the advancements in diagnosis of bacterial lower respiratory tract infections. Diagnostics, 9(2), 37. https://doi.org/10.3390/diagnostics9020037
Oktarina, R., Dewi, N. A., & Widayani, P. (2020). Machine learning approach for Acute Respiratory Infections ( ISPA ) prediction : Case study Indonesia Machine learning approach for Acute Respiratory Infections ( ISPA ) prediction : Case study Indonesia. Journal of Physics: Conf. Series, 1469. https://doi.org/10.1088/1742-6596/1469/1/012044
Pomeranz, G., Pando, R., Hindiyeh, M., Sherbany, H., Meningher, T., Sharabi, S., Kolet, L., Pomeranz, A., Mendelson, E., & Mandelboim, M. (2019). Rhinovirus infections in infants suggest that early detection can prevent unnecessary treatment. Journal of Clinical Virology, 115(March), 11–17. https://doi.org/10.1016/j.jcv.2019.03.012
Shi, T., Arnott, A., Semogas, I., Falsey, A. R., Openshaw, P., Wedzicha, J. A., Campbell, H., & Nair, H. (2020). The etiological role of common respiratory viruses in acute respiratory infections in older adults: a systematic review and meta-analysis. The Journal of Infectious Diseases, 222(Supplement_7), S563–S569. https://doi.org/10.1093/infdis/jiy662
Subramony, A., Zachariah, P., Krones, A., Whittier, S., & Saiman, L. (2016). Impact of multiplex polymerase chain reaction testing for respiratory pathogens on healthcare resource utilization for pediatric inpatients. The Journal of Pediatrics, 173, 196–201. https://doi.org/10.1016/j.jpeds.2016.02.050
Surury, I., & Azizah, M. (2022). Spatial Analysis of Acute Respiratory Infection (ARI) Based on The Air Pollution Standard Index (PSI) at DKI Jakarta Region in 2018-2019. Jurnal Kesehatan Lingkungan, 14(2), 90–98. https://doi.org/10.20473/jkl.v14i2.2022.90-98
Tran, D. N., Trinh, Q. D., Pham, N. T. K., Pham, T. M. H., Ha, M. T., Nguyen, T. Q. N., Okitsu, S., Shimizu, H., Hayakawa, S., & Mizuguchi, M. (2016). Human rhinovirus infections in hospitalized children: clinical, epidemiological and virological features. Epidemiology & Infection, 144(2), 346–354. https://doi.org/10.1017/S0950268815000953
Yanagihara, K. (2019). The role of molecular diagnosis in acute respiratory tract infection. Respiratory Investigation, 57(6), 511. https://doi.org/10.1016/j.resinv.2019.06.007
Yunus, M., Raharjo, W., & Fitriangga, A. (2020). Faktor-faktor yang berhubungan dengan kejadian infeksi saluran pernapasan akut (ISPA) pada pekerja PT. X. Jurnal Cerebellum, 6(1), 21–30.
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