Antibacterial Potential of Curcuma Mangga Kombucha: The Effect of Fermentation Duration on Activity Against Escherichia coli and Salmonella typhi
DOI:
https://doi.org/10.31964/mltj.v11i1.636Keywords:
Kombucha, Curcuma mangga, Fermentation, Antibacterial, Escherichia coli, Salmonella typhiAbstract
Kombucha is a fermented beverage known for its various health benefits, including antibacterial activity. This study aims to evaluate the antibacterial potential of mango ginger (Curcuma mangga)-based kombucha against Escherichia coli and Salmonella typhi, as well as to analyze the effect of fermentation duration on the physicochemical properties of kombucha. Fermentation was conducted for 5, 7, 10, and 14 days, with total acidity, pH, and antibacterial activity measurements using the well diffusion method. The results showed total acidity increased as fermentation progressed, while pH decreased. Antibacterial tests demonstrated that mango ginger kombucha exhibited inhibitory activity against Escherichia coli and Salmonella typhi, with the largest inhibition zones observed on day 10 of fermentation (8.2 mm for Escherichia coli and 9.2 mm for Salmonella typhi). Although its inhibitory effect was moderate compared to the positive control (ciprofloxacin), these findings suggest the potential of mango ginger kombucha as a functional beverage with antibacterial properties. Further research is needed to identify the active compounds responsible for its antimicrobial activity and to optimize the fermentation process to enhance its effectiveness.References
Adila, R., & Agustien, A. (2013). Uji Antimikroba Curcuma spp. Terhadap Pertumbuhan Candida albicans, Staphylococcus aureus dan Escherichia coli. Jurnal Biologi Universitas Andalas, 1–7.
Afifah, N. (2019). Analisis kondisi dan potensi lama fermentasi medium (teh kopi rosella) dalam menghambat pertumbuhan bakteri pathogen (Vibrio cholera dan Bacillus cereus). Unversitas Islam Negeri Malang, 1992, 7–37.
Al-Mohammadi, A. R., Ismaiel, A. A., Ibrahim, R. A., Moustafa, A. H., Zeid, A. A., & Enan, G. (2021). Chemical constitution and antimicrobial activity of kombucha fermented beverage. Molecules, 26(16), 11–13. https://doi.org/10.3390/ molecules26165026
Aulia Saudi, A. D. (2018). Uji daya hambat antibiotika terhadap bakteri penyebab infeksi saluran kemih di Rumah Sakit Salewangang Maros. Analysis Of Antibiotic Treatment On Bacteria Causing Urinary Tract Infection In Salewangang Maros. Media Farmasi, 14(2), 27. https://doi.org/10.32382/mf.v14i2.587
Barkah, Muhammad Habib, Dini Sri Damayanti, R. H. (2020). Pengaruh Lama Fermentasi Kombucha Daun Sirsak (Annona muricata L.)Terhadap Daya Hambat Pertumbuhan bakteri Escherichia coli. Repository University of Islam Malang, 1.
Battikh, H., Chaieb, K., Bakhrouf, A., & Ammar, E. (2013). Antibacterial and antifungal activities of black and green kombucha teas. Journal of Food Biochemistry, 37(2), 231–236. https://doi.org/10.1111/j.1745-4514.2011.00629.x
Bhattacharya, D., Bhattacharya, S., & Manti, M. (2016). Antibacterial Activity of Polyphenolic Fraction of Kombucha Against Enteric Bacterial Pathogens. Current Microbiology, 73(6), 885–896. https://doi.org/10.1007/s00284-016-1136-3
Budi, A., & Sembiring, N. L. (2022). Polaresistensi Bakteri Salmonella typhi Terhadap Antibiotik Ceftriaxone Dan Ciprofloxacin Polaresistency of Salmonella typhi Bacteria To Antibiotic Ceftriaxone and Ciprofloxacin. Journal Health And Science ; Gorontalo Journal Health & Science Community, 6, 58–67.
Budiari, S., Mulyani, H., Maryati, Y., Filailla, E., Devi, A. F., & Melanie, H. (2023). Chemical properties and antioxidant activity of sweetened red ginger extract fermented with kombucha culture. Agrointek : Jurnal Teknologi Industri Pertanian, 17(1), 60–69. https://doi.org/10.21107/agrointek.v17i1.14269
Cahyaningrum, P. L., & Artini, N. P. R. (2018). Uji Aktivitas Antibakteri Serbuk Instan Kombinasi Temu Mangga (Curcuma mangga Val) dan Daun Anting-Anting (Acalypha indica L.). Jurnal Kesehatan Terpadu, 2(1), 1–6. https://doi.org/10.36002/jkt.v2i1.439
CLSI. (2020). Performance standards for antimicrobial susceptibility testing. CLSI Document M100, 30th Edition. Wayne: Clinical and Laboratory Standards Institute.
de Miranda, J. F., Ruiz, L. F., Silva, C. B., Uekane, T. M., Silva, K. A., Gonzalez, A. G. M., Fernandes, F. F., & Lima, A. R. (2022). Kombucha: A review of substrates, regulations, composition, and biological properties. Journal of Food Science, 87(2), 503–527. https://doi.org/10.1111/1750-3841.16029
Dwiyati Pujimulyani, W. A. Y. (2023). Penyuluhan Manfaat Kunir Putih (Curcuma mangga Val.) dan Makanan Probiotik di Kelompok Tani Menulis. Jati Emas (Jurnal Aplikasi Teknik Dan Pengabdian Masyarakat), 7(3), 57–62.
Fauzi, M., Harsono, S. S., Nurana, Y., Pertanian, T., Program, ), Tep, S., Jalan, U., No, K., & Timur, J. J. (2023). Karakteristik Minuman Kombucha Temulawak (Curcuma xanthorrhiza Roxb) Hasil Fermentasi Pada Berbagai Konsentrasi Ekstrak dan Lama Inkubasi Characteristics of Temulawak Kombucha Drink (Curcuma xanthorrhiza Roxb) Fermentation Product at Various Extract Conce. Jipang), 5(2), 1–10.
Ivanišová, E., Meňhartová, K., Terentjeva, M., Harangozo, Ľ., Kántor, A., & Kačániová, M. (2020). The evaluation of chemical, antioxidant, antimicrobial and sensory properties of kombucha tea beverage. Journal of Food Science and Technology, 57(5), 1840–1846. https://doi.org/10.1007/s13197-019-04217-3
Jayabalan, R., Malbaša, R. V., Lončar, E. S., Vitas, J. S., & Sathishkumar, M. (2014). A Review on Kombucha Tea—Microbiology, Composition, Fermentation, Beneficial Effects, Toxicity, and Tea Fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4), 538–550.
Julia, R., Simanjuntak, D., Kurniawaty, E., Studi, P., Dokter, P., Kedokteran, F., Lampung, U., Biokima, B., Kedokteran, F., & Lampung, U. (2019). Efek Antibakteri Kopi Robusta yang Difermentasi dengan Kombucha Terhadap Salmonella typhi. J Agromedicine, 6, 83–88.
Julia, R., Simanjuntak, D., Mutiara, H., Studi, P., Dokter, P., Kedokteran, F., & Lampung, U. (2016). Pengaruh Pemberian Teh Kombucha Terhadap Pertumbuhan Salmonella typhi. Majority, 5.
Júnior, J. C. da S., Meireles Mafaldo, Í., de Lima Brito, I., & Tribuzy de Magalhães Cordeiro, A. M. (2022). Kombucha: Formulation, chemical composition, and therapeutic potentialities. Current Research in Food Science, 5(February), 360–365. https://doi.org/10.1016/j.crfs.2022.01.023
Kaewkod, T., Bovonsombut, S., & Tragoolpua, Y. (2019). Efficacy of kombucha obtained from green, oolongand black teas on inhibition of pathogenic bacteria, antioxidation, and toxicity on colorectal cancer cell line. Microorganisms, 7(12), 1–18. https://doi.org/10.3390/microorganisms7120700
Kamazeri, T. S. A. T., Samah, O. A., Taher, M., Susanti, D., & Qaralleh, H. (2012). Antimicrobial activity and essential oils of Curcuma aeruginosa, Curcuma mangga, and Zingiber cassumunar from Malaysia. Asian Pacific Journal of Tropical Medicine, 5(3), 202–209. https://doi.org/10.1016/S19957645 (12)60025-X
Kluz, M. I., Pietrzyk, K., Pastuszczak, M., Kacaniova, M., Kita, A., Kapusta, I., Zaguła, G., Zagrobelna, E., Struś, K., Marciniak-Lukasiak, K., Stanek-Tarkowska, J., Timar, A. V., & Puchalski, C. (2022). Microbiological and Physicochemical Composition of Various Types of Homemade Kombucha Beverages Using Alternative Kinds of Sugars. Foods, 11(10), 1–15. https://doi.org/10.3390/FOODS11101523
Leal, J. M., Suárez, L. V., Jayabalan, R., Oros, J. H., & Escalante-Aburto, A. (2018). A review on health benefits of kombucha nutritional compounds and metabolites. CYTA - Journal of Food, 16(1), 390–399. https://doi.org/10.1080/19476337.2017.1410499
Massoud, R., & Jafari, R. (2024). Kombucha as a Health-Beneficial Drink for Human Health Chemical Components of Kombucha. Plant Foods for Human Nutrition, 79, 251–259.
Muchtaromah, B., Safitri, Evika Sandi, P. D. F., & And, J. I. (2020). Antibacterial activities of Curcuma mangga Val. extract in some solvents to Staphylococcus aureus and Escherichia coli. AIP Conference Proceedings. https://doi.org/ 10.1063/5.0002490
Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41. https://doi.org/10.24198/jthp.v1i2.27537
Putri, F. S., Rahayu, T. P., & Fitriyati, L. (2023). Uji Aktivitas Anti Bakteri Ekstrak Etanol Kunir Putih (Curcuma mangga Val) Terhadap Bakteri Bacillus subtilis Penyebab Diare. Proceeding of The 16th University Research Colloquium 2022: Bidang MIPA dan Kesehatan, 622-634. http://repository.urecol.org/ index.php/proceeding /article/view/2483
Rahmawati, Y., Ningsih, A. W., Rahmawati, Y., Agustin, F., Rohadatul, A., Ariyani, E., D, R. A. R., Charles, I., Farmasi, D. B., Medika, U. A., Farmasi, M. S., Studi, P., Farmasi, S., Kesehatan, F. I., & Medika, A. (2023). Review Artikel : Studi Fitokimia dan Farmakologi Temu Putih (Curcuma zedoria). Journal of Pharmacy Science and Technology, 4(1), 9–16.
Saffarian, H., Rahimi, E., Khamesipour, F., & Hashemi Dehkordi, S. M. (2024). Antioxidant and antimicrobial effect of sodium alginate nanoemulsion coating enriched with oregano essential oil (Origanum vulgare L.) and Trachyspermum ammi oil (Carum cupticum) on food pathogenic bacteria. Food Science and Nutrition, 12(4), 2985–2997. https://doi.org/10.1002/fsn3.3979
Sarjono, P. R., & Mulyani, N. S. (2007). Aktivitas Antibakteri Rimpang Temu Putih (Curcuma mangga Val). Jurnal Sains & Matematika (JSM), 15(April), 89–93.
Selvaraj, S., & Gurumurthy, K. (2023). An overview of probiotic health booster-kombucha tea. Chinese Herbal Medicines, 15(1), 27–32. https://doi.org/10.1016/j.chmed.2022.06.010
Sintyadewi, P. R., Fitriani, P. P. E., Widnyani, I. A. P. A., & Indrayoni, P. (2023). Potensi Aktivitas Antibakteri Minuman Fungsional Kombucha Berbahan Dasar Bunga Kecombrang (Etlingera elatior) Berdasarkan Lamanya Waktu Fermentasi. Jurnal Biologi Tropis, 23(1), 403–410. https://doi.org/10.29303/jbt.v23i1.6126
Subagya, A. N., Zukra, R. M., & Hasanah, L. N. (2020). Manajemen Gastroenteritis pada Anak: Literature Review Management of Gastroenteritis in Children: Literature Review. Jurnal Keperawatan Klinis Dan Komunitas, 4(3), 151–157.
Sugita, P., Nihayah, R. F., Ikkbal, M., Kasmaran, A., Syahbirin, G., Ilmiawati, A., Arifin, B., & Irfana, L. (2022). Identifikasi Senyawa Fraksi Larut n -Heksana Rimpang Temu Mangga (Curcuma mangga Val) Menggunakan LC-MS / MS dan Review Potensinya sebagai Antibakteri (Identification of Compounds Soluble n -Hexane Fraction of Rhizome of Temu Mangga (Curcuma manga Val). Kovalen : Jurnal Riset Kimia, 8(2), 99–112.
Susanti, Y., A’yun, A. Q., Ansori, A., Sekaringgalih, R., Rachmach, A. N. L., & Hanum, N. S. (2023). Pelatihan Pembuatan Minuman Probiotik Teh Kombucha dengan Varian Tanaman Herbal di Desa Bagorejo - Banyuwangi. Jurnal Pengabdian Pada Masyarakat, 8(2), 410–420. https://doi.org/10.30653/jppm.v8i2.383
Tandirogang, N., Anitasari, S., Arung, E. T., Paramita, S., & Shen, Y. K. (2022). Evaluations of Antibacterial Properties of Zingiber purpureum Essential Oil Against 13 Different Gram-positive and Gram-negative Bacteria. Indonesian Biomedical Journal, 14(3), 303–308. https://doi.org/10.18585/inabj.v14i3.1967
Tyagi, P., Singh, M., Kumari, H., Kumari, A., & Mukhopadhyay, K. (2015). Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS ONE, 10(3), 1–15. https://doi.org/10.1371/journal.pone.0121313
Velićanski, A., Dragoljub, C., Markov, S. L., Šaponjac, V. T. T., & Vulić, J. J. (2014). Antioxidant and Antibacterial Activity of the Beverage lemon balm. Food Technol. Biotechnol, 52(4), 420–429.
Villarreal-Soto, S. A., Beaufort, S., Bouajila, J., Souchard, J.-P., & Taillandier, P. (2018). Understanding Kombucha Tea Fermentation A Review. In Journal of Food Science (Vol. 83, pp. 580–588).
W., D. W., & R., S. T. (1971). Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology, 22(4), 659–665. https://doi.org/10.1128/am.22.4.659-665.1971
Zofia, N. Ł., Aleksandra, Z., Tomasz, B., Martyna, Z. D., Magdalena, Z., Zofia, H. B., & Tomasz, W. (2020). Effect of fermentation time on antioxidant and anti-ageing properties of green coffee kombucha. Molecules, 25(22). https://doi.org/ 10.3390/molecules25225394
Zubaidah, E., Dea, E. C., & Sujuti, H. (2022). Physicochemical and microbiological characteristics of kombucha based on various concentration of Javanese turmeric (Curcuma xanthorrhiza). Biocatalysis and Agricultural Biotechnology, 44(May), 102467. https://doi.org/10.1016/j.bcab.2022.102467
Zubaidah, E., Nisak, Y. K., Wijayanti, S. A., & Christianty, R. A. (2021). Characteristic of microbiological, chemical, and antibacterial activity of turmeric (Curcuma longa) kombucha. IOP Conference Series: Earth and Environmental Science, 924(1). https://doi.org/10.1088/1755-1315/924/1/012080.
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