Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli
DOI:
https://doi.org/10.31964/mltj.v0i0.420Keywords:
Sengkuang Leaf Extract, Escherichia coli, Minimum Inhibitory Concentration, Minimum Bactericidal ConcentrationAbstract
Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.References
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Falah, F., Sayektiningsih, T., & Noorcahyati, N. (2013). Keragaman jenis dan pemanfaatan tumbuhan berkhasiat obat oleh masyarakat sekitar Hutan Lindung Gunung Beratus, Kalimantan Timur. Jurnal Penelitian Hutan dan Konservasi Alam, 10(1), 1-18.
Fang SX., Liang, Z. Y., & Zhang, Y. X. (2008). Study the chemical constituents of essential oil from the skins of the stem of Dracontomelon dao (Blanco) Merr. & Rofe Merr. Et Rolfe. Lishizhen Medicine and Materia Medica Research, 7, 045.
Ismail S (2015). Isolasi Dan Penentuan Struktur Serta Mekanisme Aksi Senyawa Vasodilator Dari Daun Dracontonelon Dao (Blanco) Merr. & Rolfe (Dahu) Secara In Vitro (Doctoral dissertation, Universitas Gadjah Mada).
Kamath, N., Swaminathan, R., & Desai, N. (2016). Antibacterial activity of Indian medicinal plant Moringa oleifera against MRSA and Klebsiella spp.(ESBL), which are commonly isolated bacteria in hospital environments. International Journal of Applied Research, 2(8), 515-517.
Kurniawan, D., Yuliawati, R., Habibi, M., & Ramlan, E. E. (2019). Efektivitas Ekstrak Ethanol Daun Sengkuang (Dracontomelon dao) Sebagai Larvasida Alami. Jurnal Penelitian Ekosistem Dipterokarpa, 5(2), 79-86.
Li, Y., Xia, H., Wu, M., Wang, J., Lu, X., Wei, S., ... & Xiao, X. (2017). Evaluation of the antibacterial effects of flavonoid combination from the leaves of Dracontomelon dao by microcalorimetry and the quadratic rotary combination design. Frontiers in pharmacology, 8, 70.
Liu, S., Zhao, Y., Zeng, N., Liu, T., Zhang, Y., Han, B., ... & Xiao, X. (2014). Antibacterial effect of four extracts from leaves of Dracontomelon dao on Escherichia coli growth using microcalorimetry coupled with principal component analysis. Journal of Thermal Analysis and Calorimetry, 116(1), 491-497.
Ningrum, H. P., Yeni, L. F., & Ariyati, E. (2011). Uji Daya Antibakteri Ekstrak Sawo Manila Terhadap E. coli dan Implemantasinya dalam Pembelajaran Peranan Bakteri. Jurnal Pendidikan dan Pembelajaran Khatulistiwa, 2(9).
Pajan, S. A. (2016). Potensi antibakteri air perasan bawang putih (Allium sativum L) terhadap pertumbuhan Staphylococcus aureus. Pharmacon, 5(4).
Priya. 2016. Sengkuang Menjadi Obyek Penelitian Bagi Teknisi di Balitek KSDA. Badan penelitian, pengembangan dan inovasi ; Kementrian LH dan kehutanan https://www.forda-mof.org/index.php/berita/post/3046.
Putri, T. D. Y., Dharmono, D., & Utami, N. H. (2022). Kajian Etnobotani Tumbuhan Dracontomelon dao (Blanco) Merr. & Rofe (Dracontomelon dao) di Desa Sabuhur Kecamatan Jorong Kabupaten Tanah Laut Sebagai Buku Ilmiah Populer. JUPEIS: Jurnal Pendidikan dan Ilmu Sosial, 1(2), 33-42.
Sani, R. N., Nisa, F. C., Andriani, R. D., & Maligan, J. M. (2013). Analisis rendemen dan skrining fitokimia ekstrak Ethanol mikroalga laut Tetraselmis chuii [in press april 2014]. Jurnal Pangan dan Agroindustri, 2(2), 121-126.
Wulandari, A. R., Dianingati, R. S., & Sunnah, I. (2021). Optimasi Pelarut Terhadap Parameter Spesifik Ekstrak Kitolod (Isotoma Longiflora). Generics: Journal Of Research In Pharmacy, 1(1).
Zhao, Y., Liu, S., Qu, F., Wang, J., Hu, Y., Zhang, P., ... & Xiao, X. (2015). Microcalorimetry coupled with principal component analysis for investigating the anti-Staphylococcus aureus effects of different extracted fractions from Dracontomelon dao. Journal of Thermal Analysis and Calorimetry, 120(1), 913-920.
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Published
2022-06-27
How to Cite
Dwiyanti, R. D., & Nurlailah, N. (2022). Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli. Medical Laboratory Technology Journal, 8(1), 77–83. https://doi.org/10.31964/mltj.v0i0.420
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