Prevalence of Anemia among Blood Donors in Benin City: Cross-Sectional Study of Iron Status and Hematological Parameters

Authors

  • Fidelis Ohiremen Oyakhire Department of Medical Laboratory Science, Faculty of Applied Health Sciences, Edo State University Iyamho, Uzairue, Edo State, Nigeria
  • Adebukola Adisa Ekoh-Jolly Department of Medical Laboratory Science, University of Medical Sciences, Ondo, Ondo State, Nigeria
  • Babatunde Ishola Gabriel Adejumo Department of Medical Laboratory Science, University of Benin, Benin City, Edo State, Nigeria
  • Samson Efenarhua Department of Natural Science, Faculty of Science and Technology, Middlesex University, London, United Kingdom
  • Osamwonyi Ernest Igbinovia Department of Laboratory Medicine, Swansea Bay University Healthboard, Wales, United Kingdom

DOI:

https://doi.org/10.31964/mltj.v12i1.726

Keywords:

Anemia, blood donors, donor management, ferritin, hemoglobin

Abstract

Despite the importance of blood donation in ensuring a constant supply of blood for emergency situations and surgical procedures, frequent blood donation leads to iron depletion and anemia, posing significant health risks to donors. The study aimed to evaluate the iron status and hematological parameters among blood donors in Benin City. A cross-sectional study involving 100 blood donors was conducted across blood donation facilities in Benin City. Hematological parameters and ferritin levels were analyzed using an automated hematology analyzer (BK3100, China) and enzyme-linked immunosorbent assay (ELISA), respectively. Data were analyzed using SPSS version 26.0. Chi-square, one-way ANOVA, and Pearson correlation tests were applied, with significance set at p < 0.05. Results of the participants, 64.0% were males and 36.0% females. The self-reported prevalence of anemia was 1.0%, while all participants had hemoglobin values below WHO reference cutoffs (p<0.05). Male donors showed significantly higher hemoglobin (12.37±0.23 vs 11.05±0.17 g/dL), packed cell volume (37.43±0.60 vs 33.20±0.48%), and ferritin levels (180.23±12.79 vs 117.48±17.43 ng/mL) compared to females (p < 0.05). Conversely, white blood cell and platelet counts were significantly higher in females (p < 0.05). No significant differences were observed across age groups and donation frequencies. Blood donation frequency showed weak negative, non-significant correlations with hemoglobin, packed cell volume, and ferritin levels (p > 0.05). Conclusion Despite the prevalence found in this study, these findings underscore the need to monitor hematological parameters and ferritin levels in blood donors, particularly among women, to ensure donor health and maintain a safe and adequate blood supply.

References

Baidoo, B., Benya, G., Ankomah, E., Adu, P., Obike, E., Alhassan, M., Boachie, J., & Benfo, A. (2024). Mixed-methods exploration of the knowledge of young adults about blood donation processes: A one-center cross-sectional study in a tertiary institution. PLOS ONE, 19(1), e0295600. https://doi.org/10.1371/journal.pone. 0295600

Borai, A., Ichihara, K., Bahijri, S., Almasoud, A., Tamimi, W., Abdulhadi, W., Lingga, J., Bawazeer, A., Abdelaal, M., Boraie, S., Alsofyani, A., Elsayid, M., Sannan, N. S., Al-Shareef, A. S., Khan, E., & Almohammadi, M. (2023). Establishment of reference intervals for hematological parameters of adult population in the western region of Saudi Arabia. PLOS ONE, 18(2), e0281494. https://doi.org/10.1371/journal.pone.0281494

Didriksen, M., Pedersen, O. B., Ullum, H., Burgdorf, K., Sørensen, E., Mikkelsen, S., Larsen, M. A. H., Thørner, L. W., Hjalgrim, H., Erikstrup, C., Bruun, M. T., Nielsen, K. R., Banasik, K., & Rostgaard, K. (2021). The impact of health-related quality of life and depressive symptoms on blood donor career: Results from the Danish blood donor study. Transfusion, 61(5), 1479–1488. https://doi.org/10.1111/trf.16336

Drechsler, L. Ø., Erikstrup, C., Boldsen, J. K., Rostgaard, K., Ostrowski, S. R., Schwinn, M., Hjalgrim, H., Aagaard, B., Mikkelsen, C., Harritshøj, L. H., Mikkelsen, S., Pedersen, O. B., Brøns, N., Hindhede, L., Brodersen, T., & Topholm Bruun, M. (2023). The effect of ferritin-guided iron supplementation among Danish female first-time blood donors. Transfusion, 63(9), 1710–1718. https://doi.org/10.1111/trf.17484

Ekroos, S., Karregat, J., Toffol, E., Castrén, J., Arvas, M., & van den Hurk, K. (2024). Menstrual blood loss is an independent determinant of hemoglobin and ferritin levels in premenopausal blood donors. Acta Obstetricia et Gynecologica Scandinavica, 103(8), 1645–1656. https://doi.org/10.1111/aogs.14890

Fransen, M., Simon, T., Hershman, J., McCausland, K., Parfitt, A., Lenart, J., Weissfeld, L., & Becker, M. (2023). Effects of donation frequency on U.S. source plasma donor health. Transfusion, 63(10), 1885–1903. https://doi.org/10.1111/trf.17523

Gasbarrino, K., Zheng, H., & Daskalopoulou, S. S. (2024). Circulating sex-specific markers of plaque instability in women and men with severe carotid atherosclerosis. Stroke, 55(2), 269–277. https://doi.org/10.1161/STROKEAHA.123.044840

Karregat, J., Sweegers, M. G., Quee, F. A., Weekamp, H. H., Swinkels, D. W., Novotny, V. M. J., Zaaijer, H. L., & van den Hurk, K. (2022). Ferritin-guided iron supplementation in whole blood donors: Optimal dosage, donor response, return and efficacy (FORTE)—A randomized controlled trial protocol. BMJ Open, 12(3), e056316. https://doi.org/10.1136/bmjopen-2021-056316

Kreft, I. C., Prinsze, F., van Alphen, F. P. J., Hoogendijk, A. J., van den Hurk, K., de Korte, D., van der Zwaan, C., Meijer, A. B., Boon-Spijker, M., & van den Biggelaar, M. (2023). Mass spectrometry-based analysis on the impact of whole blood donation on the global plasma proteome. Transfusion, 63(3), 564–573. https://doi.org/10.1111/trf.17254

Mantadakis, E., Martinis, G., Kontekaki, E., Bezirgiannidou, Z., & Panagopoulou, P. (2022). Iron deficiency and blood donation: Links, risks and management. Journal of Blood Medicine, 13, 775–786. https://doi.org/10.2147/JBM.S375945

Moazzen, S., Sweegers, M. G., Janssen, M., Hogema, B. M., Hoekstra, T., & van den Hurk, K. (2022). Ferritin trajectories over repeated whole blood donations: Results from the FIND+ study. Journal of Clinical Medicine, 11(13), 3581. https://doi.org/10.3390/jcm11133581

Müller, H. M., & Reuter-Oppermann, M. (2024). Designing behavior change support systems targeting blood donation behavior. Business & Information Systems Engineering, 66(3), 299–319. https://doi.org/10.1007/s12599-024-00878-3

Paalvast, Y., van den Hurk, K., Janssen, M., Sweegers, M., Moazzen, S., & Hogema, B. (2022). A computational model for prediction of ferritin and haemoglobin levels in blood donors. British Journal of Haematology, 199(1), 143–152. https://doi.org/10.1111/bjh.18367

Prinsze, F. J., Zalpuri, S., de Groot, R., van den Hurk, K., & Timmer, T. C. (2021). Donation-induced iron depletion is significantly associated with low hemoglobin at subsequent donations. Transfusion, 61(12), 3344–3352. https://doi.org/10.1111/trf.16688

Ruzhanskaya, A., Sukhacheva, E., Ichihara, K., Vybornova, N., Lugovskaya, S., Agarkova, G., Emanuel, V., Vilenskaya, E., Skibo, I., Butlitski, D., & Vasiliev, A. (2024). Derivation of Russian-specific reference intervals for complete blood count, iron markers and related vitamins. PLOS ONE, 19(9), e0304020. https://doi.org/10.1371/journal.pone.0304020

Senefeld, J. W., & Hunter, S. K. (2024). Hormonal basis of biological sex differences in human athletic performance. Endocrinology, 165(5), bqae036. https://doi.org/10.1210/endocr/bqae036

Sheldon, K. M., Titova, L., Osin, E., Khrushev, S., Vybornykh, D., Lapka, S., Gaponova, T., & Rasskazova, E. (2022). Blood donation motivation in the United States and Russia: What keeps donors returning? Journal of Community & Applied Social Psychology, 32(5), 872–881. https://doi.org/10.1002/casp.2608

Sheykhsoltan, M., Ziman, A., Wu, W., Mei, Z., & Ward, D. C. (2021). Who donates? Patterns of blood donation and donor characteristics at a university-affiliated hospital-based donor center. Transfusion, 62(2), 346–354. https://doi.org/10.1111/trf.16754

Spencer, B. R., & Mast, A. E. (2022). Iron status of blood donors. Current Opinion in Hematology, 29(6), 310–316. https://doi.org/10.1097/MOH.0000000000000733

Thuer, L., Ahrens, N., Brosig, A., Hähnel, V., Offner, R., Hutchinson, J. A., & Burkhardt, R. (2021). Total platelet donation count and donation frequency are determinants of plateletpheresis-associated lymphopenia. Transfusion, 61(11), 3161–3173. https://doi.org/10.1111/trf.16666

Tsurusawa, N., Chang, J., Namba, M., Makioka, D., Yamura, S., Iha, K., Kyosei, Y., Watabe, S., Yoshimura, T., & Ito, E. (2021). Modified ELISA for ultrasensitive diagnosis. Journal of Clinical Medicine, 10(21), 5197. https://doi.org/10.3390/jcm10215197

van den Hurk, K., Arvas, M., Roberts, D. J., Castrén, J., & Erikstrup, C. (2024). Whole blood donor iron management across Europe: Experiences and challenges in four blood establishments. Transfusion Medicine Reviews, 38(4), 150860. https://doi.org/10.1016/j.tmrv.2024.150860

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Published

2026-06-30

How to Cite

Oyakhire, F. O., Ekoh-Jolly, A. A., Adejumo, B. I. G. ., Efenarhua, S. ., & Igbinovia, O. E. . (2026). Prevalence of Anemia among Blood Donors in Benin City: Cross-Sectional Study of Iron Status and Hematological Parameters. Medical Laboratory Technology Journal, 12(1), 108–120. https://doi.org/10.31964/mltj.v12i1.726

Issue

Vol. 12 No. 1 (2026): June

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Articles

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Copyright (c) 2026 Fidelis Ohiremen Oyakhire, Adebukola Adisa Ekoh-Jolly, Babatunde Ishola Gabriel Adejumo, Samson Efenarhua, Osamwonyi Ernest Igbinovia

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