Phytochemical Analysis by LC-HRMS and Antibacterial Activity Of the Ethanol Extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe)
DOI:
https://doi.org/10.31964/mltj.v9i1.506Keywords:
Dracontomelon dao (Blanco) Merr. & Rofe, Phytochemical Analysis, Liquid Chromatography - High-Resolution Mass SpectrometryAbstract
Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants for the community. This study aims to analyze the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) using the LC-HRMS Phytochemical Test and determine its antibacterial activity. Leaf Dracontomelon dao (Blanco) Merr. & Rofe originates from Hulu Sungai Utara, South Kalimantan, Indonesia, macerated with 96% ethanol. Antibacterial test by diffusion was carried out using the well method. LC-HRMS (Liquid Chromatography - High-Resolution Mass Spectrometry) test using the Instrument Thermo Scientific™ Dionex™ Ultimate 3000 RSLCnano UHPLC coupled with Thermo Scientific™ Q Exactive™ High-Resolution Mass Spectrometer. The content of chemical compounds from the ethanol extract, namely flavonoids 125.5 ± 0.433 mgEQ/g, alkaloids (%) 33.945 ± 0.781, saponins (%) 33.093 ± 0.755, tannins (mg/ml) 0.069 ± 0.003, the highest content was triterpenoids 669.8 ± 2,000(mg/ml). The best inhibition of E coli growth with the largest inhibition zone was 24 mm at a 500 mg/ml concentration. The active compounds contained in the leaves of Dracontomelon dao (Blanco) Merr. & Rolfe are quercetin-3β-D-glucoside, anacardic acid, D-glucosamine, azelaic acid, choline, astragalin, quercetin, luteolin, syringic acid. The active ingredient of the ethanol extract of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) has the potential as an antibacterial and anti-inflammatory that can be added to pharmaceutical preparations. Keywords: Dracontomelon dao (Blanco) Merr. & Rofe; Phytochemical Analysis; Liquid Chromatography - High-Resolution Mass SpectrometryReferences
Akroum, S., Bendjeddou, D., Satta, D., and Lalaoui, K. (2009). Antibacterial activity and acute toxicity effect of flavonoids extracted from Mentha longifolia. Am. Eurasia. J. Sci. Res. 4, 93–96.
Eumkeb, G., Siriwong, S., and Thumanu, K. (2012). Synergistic activity of luteolin and amoxicillin combination against amoxicillin-resistant Escherichia coli and mode of action. J. Photochem. Photobiol. B 117, 247–253. doi 10.1016/j.jphotobiol.2012.10.006
Falah, F., Sayektiningsih, T., & Noorcahyati, N. (2013). Keragaman jenis dan pemanfaatan tumbuhan berkhasiat obat oleh masyarakat sekitar Hutan Lindung Gunung Beratus, Kalimantan Timur. Jurnal Penelitian Hutan dan Konservasi Alam, 10(1), 1-18.
Bladon PT. (1986). Topical azelaic acid and the treatment of acne: a clinical and laboratory comparison with oral tetracycline. Brit. J. Dermatol, 114:493–499. doi 10.1111/j.1365-2133.1986.tb02856.x.
Blaskovich MAT, Elliott AG, Kavanagh AM, Ramu S, Cooper MA. (2019) In vitro Antimicrobial Activity of Acne Drugs Against Skin-Associated Bacteria. Sci Rep, 9(1):14658. Doi: 10.1038/s41598-019-50746-4.
Dwiyanti RD, Nurlailah. (2022). Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli, Medical Laboratory Technology Journal, 8(1), 77-83
Hu X, Wang M, Pan Y, Xie Y, Han J, Zhang X, Niayale R, He H, Li Q, Zhao T, Cui Y, Yu S. (2020). Anti-inflammatory Effect of Astragalin and Chlorogenic Acid on Escherichia coli-Induced Inflammation of Sheep Endometrial Epithelium Cells. Front Vet Sci.7: 201. Doi: 10.3389/fvets.2020.00201
Ibsen KN, Ma H, Banerjee A, Tanner EEL, Nangia S, Mitragotri S. (2018). Mechanism of Antibacterial Activity of Choline-Based Ionic Liquids (CAGE). ACS Biomater Sci Eng, 4(7):2370–2379. doi: 10.1021/acsbiomaterials.8b00486.
Joanne L. Gellerman, Nancy J. Walsh, Nancy K. Werner, and Hermann Schlenk. (2011). Antimicrobial effects of anacardic acids. Canadian Journal of Microbiology. 15(10): 1219-1223. https://doi.org/10.1139/m69-220
Khan, M. R., and Omoloso, A. D. (2002). Antibacterial and antifungal activities of Dracontomelon dao. Fitoterapia 73, 327–330. doi: 10.1016/S0367-326X(02)00076-X
Kurniawan, D., Yuliawati, R., Habibi, M., & Ramlan, E. E. (2019). Efektivitas Ekstrak Ethanol Daun Sengkuang (Dracontomelon dao) Sebagai Larvasida Alami. Jurnal Penelitian Ekosistem Dipterokarpa, 5(2), 79-86.
Li, Y., Xia, H., Wu, M., Wang, J., Lu, X., Wei, S., & Xiao, X. (2017). Evaluation of the antibacterial effects of flavonoid combination from the leaves of Dracontomelon dao by microcalorimetry and the quadratic rotary combination design. Frontiers in pharmacology, 8, 70.
Liu, T., Zhao, Y. L., Wang, J. B., Zhou, X., Sun, Z., Zheng, Q. (2013). The action of crude Radix Aconiti Lateralis (Fuzi) and its processed products on splenic lymphocyte growth investigated by microcalorimetry. Therm. Acta 571, 1–7. doi 10.1016/j.tca.2013.07.031
Lv, P. C., Li, H. Q., Xue, J. Y., Shi, L., and Zhu, H. L. (2009). Synthesis and biological evaluation of novel luteolin derivatives as antibacterial agents. Eur. J. Med. Chem. 44, 908–914. doi: 10.1016/j.ejmech.2008.01.013
Matsumura S, Kawamura Y, Yoshikawa S, Kawada K, Uchibori T. (1993). Surface activities, biodegradability and antimicrobial properties of glucosamine derivatives containing alkyl chains, Journal AOCS, 70(1): 17-22. https://doi.org/10.1007/BF02545361
Minich A, Levarski Z, Mikulášová M, Straka M, Liptáková A, Stuchlík S. (2022). Complex Analysis of Vanillin and Syringic Acid as Natural Antimicrobial Agents against Staphylococcus epidermidis Biofilms. Int J Mol Sci, 23(3):1816. doi: 10.3390/ijms23031816.
Nazzaro-Porro M. (1983). Beneficial effect of 15% azelaic acid cream on acne vulgaris. Brit. J. Dermatol, 109:45–48. doi: 10.1111/j.1365-2133.1983.tb03990.x.
Riaz A, Rasul A, Hussain G, Zahoor MK, Jabeen F, Subhani Z, Younis T, Ali M, Sarfraz I, Selamoglu Z. (2018). Astragalin: A Bioactive Phytochemical with Potential Therapeutic Activities. Adv Pharmacol Sci, 2018: 9794625. Doi: 10.1155/2018/9794625.
Saedtler M, Förtig N, Ohlsen K, Faber F, Masota N, Kowalick K, Holzgrabe U, Meinel L. (2020). Antibacterial Anacardic Acid Derivatives. ACS Infect Dis, 6(7): 1674–1685. doi: 10.1021/acsinfecdis.9b00378
Skarbek K, Gabriel I, Szweda P, Wojciechowski M, Khan MA, Görke B, Milewski S, Milewska MJ. (2017). Synthesis and antimicrobial activity of 6-sulfo-6-deoxy-D-glucosamine and its derivatives. Carbohydr Res, 448: 79-87. doi: 10.1016/j.carres.2017.06.002.
Wu, M. Q., Qu, F., Zhao, Y. L., Wang, J. B., Su, H., Chen, C. (2015).Microcalorimetry and turbidimetry to investigate the antibacterial activities of five fractions from the leaves of Dracontomelon dao on P. aeruginosa. J. Therm. Anal. Calorimetry 123, 2367–2376. doi: 10.1007/s10973-015-4932-2
Zhao, Y. L., Liu, S. X., Qu, F., Wang, J. B., Hu, Y., Zhang, P.(2015). Microcalorimetry coupled with principal component analysis for investigating the anti-Staphylococcus aureus effects of different extracted fractions from Dracontomelon dao. J. Therm Anal. Calorimetry 120, 913–920. doi: 10.1007/s10973-014-4268-3
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Ratih Dewi Dwiyanti, Anny Thuraidah, Nurlailah Nurlailah
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Publishing your paper with Medical Laboratory Technology Journal (MLTJ) means that the author or authors retain the copyright in the paper. MLTJ granted an author(s) rights to put the paper onto a website, distribute it to colleagues, give it to students, use it in your thesis etc, even commercially. The author(s) can reuse the figures and tables and other information contained in their paper published by MLTJ in future papers or work without having to ask anyone for permission, provided that the figures, tables or other information that is included in the new paper or work properly references the published paper as the source of the figures, tables or other information, and the new paper or work is not direct at private monetary gain or commercial advantage.
MLTJ journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This journal is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets others remix, transform, and build upon the material for any purpose, even commercially. MLTJ journal Open Access articles are distributed under this Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA). Articles can be read and shared for All purposes under the following conditions:
BY: You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.SA: If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
